Nervous and muscle systems of Bryozoa

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The collection contains images of temporary preparations of 3 species of freshwater bryozoans from different families of the order Plumatellida (class Phylactolaemata). The images were taken by confocal laser scanning microscopy. The preparations were created using immunohistochemical staining of nervous elements for serotonin, FMRFamide and alpha-tubulin and histochemical staining of catecholamine-reactive neurons. Muscles were stained with phalloidin and some preparations were stained with Hoechst. The collection includes images of the central and peripheral nervous systems of the animals, and of the nervous system of polypides and cystides. There are also images of the muscular system, innervation of the colonial base, and neuromuscular interactions in the lophophore and cystide of the zooids. The collection is kept on the server of the Zoological Institute RAS (Laboratory of Evolutionary Morphology) and is available from the author.

Localization of FMRFamide-immunoreactive nerve cells in the nervous system of a zooid of the bryozoan Fredericella sultana.

 

Collection author(s): K.V. Shunklina

Place of storage: Zoological Institute RAS

Taxa: 

  1. Cristatella mucedo Cuvier 1798, fam. Cristatellidae
  2. Plumatella repens Linnaeus 1758, fam. Plumatellidae
  3. Fredericella sultana Blumenbach 1779, fam. Fredericellidae

 

Preparations

Preparation ZIN-KVS-0-1

Whole mount of Cristatella mucedo zooid, stained with antibodies to 5HT and with phalloidin.

Species

Cristatella mucedo Cuvier, 1798

Taxon

Bryozoa

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Description: Whole-mount preparation of a zooid of Cristatella mucedo stained with phalloidin and antibodies to 5HT.

Staining: F-actin in muscle fibers (Phalloidin), serotonin

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Preparation ZIN-KVS-0-2

FMRFamidergic nervous system of the lophophore and cystid of the freshwater bryozoan Plumatella repens (Bryozoa, Phylactolaemata).

Species

Plumatella repens (Linnaeus, 1758)

Taxon

Bryozoa

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Description: Total preparation of a Plumatella repens zooid, stained with FMRFamide antibody and phalloidin.

Staining: F-actin in muscle fibers (Phalloidin), FMRFamide

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Preparation ZIN-KVS-0-3

Serotonergic nervous system of the lophophore of the freshwater bryozoan Fredericella sultana (Bryozoa, Phylactolaemata)

Species

Fredericella sultana Blumenbach, 1779

Taxon

Bryozoa

  More information...    

Description: Whole mount of a Fredericella sultana zooid, stained with 5HT, α-tubulin antibodies and phalloidin.

Staining: alpha-tubulin, F-actin in muscle fibers (Phalloidin), serotonin

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Preparation ZIN-KVS-0-5

Serotonergic nervous system and the musculature of the lophophore of the freshwater bryozoan Plumatella repens (Bryozoa, Phylactolaemata).

Species

Plumatella repens (Linnaeus, 1758)

Taxon

Bryozoa

  More information...    

Description: Whole mount of a Plumatella repens zooid, stained with antibodies to 5HT and with phalloidin.

Staining: F-actin in muscle fibers (Phalloidin), serotonin

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Preparation ZIN-KVS-0-6

FMRFamidergic part of the cerebral ganglion of Cristatella mucedo (Bryozoa, Phylactolaemata)

Species

Cristatella mucedo Cuvier, 1798

Taxon

Bryozoa

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Description: Whole mount of Cristatella mucedolophophore base, stained with antibodies to fMRF and a-tubulin.

Staining: alpha-tubulin, FMRFamide

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Preparation ZIN-KVS-0-7

FMRFamidergic nervous system in the body wall of a polypide of Plumatella repens (Bryozoa, Phylactolaemata).

Species

Plumatella repens (Linnaeus, 1758)

Taxon

Bryozoa

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Description: Whole mount of Plumatella repens polypide, stained with antibodies to FMRF and a-tubuline and with falloidin.

Staining: alpha-tubulin, F-actin in muscle fibers (Phalloidin), FMRFamide

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Preparation ZIN-KVS-0-8

Nerve fibers of the lophophore of Fredericella sultana stained with antibodies to alpha-tubulin.

Species

Fredericella sultana Blumenbach, 1779

Taxon

Bryozoa

  More information...    

Description: Whole mount of Fredericella sultana lophophore, stained with antibodies to a-tubulin.

Staining: alpha-tubulin

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