Preparation

Whole mount of Cristatella mucedo zooid, stained with antibodies to 5HT and with phalloidin.

In
Collection 7

Collection
Number

ZIN-KVS-0-1

Species: Cristatella mucedo Cuvier, 1798    Bryozoa

Preparation Type: whole mount

Prepared: K.V. Shunklina

Description: Whole-mount preparation of a zooid of Cristatella mucedo stained with phalloidin and antibodies to 5HT.

Method: F-actin in muscle fibers (Phalloidin), serotonin

Method Description: The staining method and protocol are described in detail in: Shunkina, K. V., Zaytseva, O. V., Starunov, V. V., & Ostrovsky, A. N. (2015). Comparative morphology of the nervous system in three phylactolaemate bryozoans. Frontiers in zoology, 12(1), 28. http://www.frontiersinzoology.com/content/12/1/28

Institution: Zoological Institute RAS

 

 

 

Series
of images

Serotonergic nervous system and musculature of Cristatella mucedo zooid.

Preparation

ZIN-KVS-0-1

Description: Cristatella mucedo. Bottom view of the anal portion of the lophophore. Central nervous system of a zooid stained with antibodies to serotonin. The series shows the shape of the serotonin-positive part of the cerebral ganglion and large nerve cords innervating the horns of the lophophore.

Organs: сentral nervous system, cerebral brain or its part, ganglion / ganglia, neural tract, peripheral nervous system, musculature innervation / musculature

Methods: Immunohistochemistry - serotonin,
Histochemistry - F-actin in muscle fibers (Phalloidin)

Series Details...