Preparation

FMRFamidergic nervous system of the lophophore and cystid of the freshwater bryozoan Plumatella repens (Bryozoa, Phylactolaemata).

In
Collection 7

Collection
Number

ZIN-KVS-0-2

Species: Plumatella repens (Linnaeus, 1758)    Bryozoa

Preparation Type: whole mount

Prepared: K.V. Shunklina

Description: Total preparation of a Plumatella repens zooid, stained with FMRFamide antibody and phalloidin.

Method: F-actin in muscle fibers (Phalloidin), FMRFamide

Method Description: The staining method and protocol are described in detail in: Shunkina, K. V., Zaytseva, O. V., Starunov, V. V., & Ostrovsky, A. N. (2015). Comparative morphology of the nervous system in three phylactolaemate bryozoans. Frontiers in zoology, 12(1), 28. http://www.frontiersinzoology.com/content/12/1/28

Institution: Zoological Institute RAS

 

 

 

Series
of images

Total preparation of a Plumatella repens zooid, stained with FMRFamide antibody and phalloidin.

Preparation

ZIN-KVS-0-2

Description: Plumatella repens. Visceral and peripheral nervous systems at the base of the lophophore and in the wall of the cystide stained with antibodies to FMRFamide. The series shows that the majority of nerve fibers run within the intestinal wall and the wall of the cystide parallel to the apico-basal axis of the zooid. The preparations stained with phalloidin show the differences in muscle arrangement in different regions of the zooid and in orientation of muscle fibers in the walls of the cystide and intestine.

Organs: neural tract, peripheral nervous system, visceral nervous system

Methods: Histochemistry - F-actin in muscle fibers (Phalloidin),
Immunohistochemistry - FMRFamide

Series Details...