Optical
slice
series

Whole mount of Plumatella repens polypide, stained with antibodies to FMRF and a-tubuline and with falloidin.

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Preparation

ZIN-KVS-0-7

Storage
place

ZIN RAS

Collection: Nervous and muscle systems of Bryozoa

Species: Plumatella repens (Linnaeus, 1758)    Bryozoa

Description: Preparation of a polypide of Plumatella repens stained with phalloidin and with antibodies to FMRFamide and tubulin showing multipolar neurons located in the nerve plexus of the body wall and the transverse fibers of the musculature. The lophophore is inside the polypide.

Comments: none

Organs: peripheral nervous system, neuron(s), receptor cell(s), musculature innervation / musculature

Methods: Immunohistochemistry - FMRFamide,
Immunohistochemistry - alpha-tubulin,
Histochemistry - F-actin in muscle fibers (Phalloidin)

Publications: Шунькина К.В., Старунов В.В., Зайцева О.В., Островский А.Н. Серотонин и FMRFамид- иммунореактивные элементы в нервной системе пресноводных мшанок (Bryozoa: Phylactolaemata) // Доклады Академии Наук. - 2013. - Т. 451. - №4. - С. 474-477. Шунькина К.В., Старунов В.В., Зайцева О. В., Островский А.Н. Сравнительная нейроморфология лофофора и стенки тела трех видов пресноводных мшанок (Bryozoa, Phylactolaemata) //Зоологический журнал. - 2014. - T. 93. - №3. - С. 497-507.Shunkina K.V., Zaytseva O.V., Starunov V.V., Ostrovsky A.N. Comparative morphology of the nervous system in three phylactolaemate bryozoans // Frontiers in Zoology 2015, 12:28 doi:10.1186/s12983-015-0112-2

Microscope: Leica TCS SPE

Stacks: 

green - FMRFamide
violet - alpha-tubulin
Red - phalloidin
Green - FMRFamide, red - phalloidin

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