Preparation

FMRFamidergic nervous system in the body wall of a polypide of Plumatella repens (Bryozoa, Phylactolaemata).

In
Collection 7

Collection
Number

ZIN-KVS-0-7

Species: Plumatella repens (Linnaeus, 1758)    Bryozoa

Preparation Type: whole mount

Prepared: K.V. Shunklina

Description: Whole mount of Plumatella repens polypide, stained with antibodies to FMRF and a-tubuline and with falloidin.

Method: alpha-tubulin, F-actin in muscle fibers (Phalloidin), FMRFamide

Method Description: The staining method and protocol are described in detail in: Shunkina, K. V., Zaytseva, O. V., Starunov, V. V., & Ostrovsky, A. N. (2015). Comparative morphology of the nervous system in three phylactolaemate bryozoans. Frontiers in zoology, 12(1), 28. http://www.frontiersinzoology.com/content/12/1/28

Institution: Zoological Institute RAS

 

 

 

Series
of images

Whole mount of Plumatella repens polypide, stained with antibodies to FMRF and a-tubuline and with falloidin.

Preparation

ZIN-KVS-0-7

Description: Preparation of a polypide of Plumatella repens stained with phalloidin and with antibodies to FMRFamide and tubulin showing multipolar neurons located in the nerve plexus of the body wall and the transverse fibers of the musculature. The lophophore is inside the polypide.

Organs: peripheral nervous system, neuron(s), receptor cell(s), musculature innervation / musculature

Methods: Immunohistochemistry - FMRFamide,
Immunohistochemistry - alpha-tubulin,
Histochemistry - F-actin in muscle fibers (Phalloidin)

Series Details...