Nervous system of annelid and mollusk larvae

The collection contains images of the nervous and muscle systems of larvae of annelids and molluscs. The images were taken by confocal laser scanning microscopy from temporary whole-mount preparations of larvae at different developmental stages. The collection includes preparations stained with antibodies against serotonin, FMRFamide, acetylated alpha-tubulin and preparations stained histochemically by phalloidin for muscles. The collection is available from the authors.

Larva (trochophore) of the annelid Phyllodoce maculata (Polychaeta, Phyllodocidae). Immunocytochemical staining of the ciliary apparatus (red) and 5-HT nervous elements (green). Photo by L.P. Nezlin.

Collection author(s): L.P. Nezlin, E.E. Voronezhskaya

Place of storage: Koltzov Institute of Developmental Biology RAS

Taxa:

Phyllodoce maculata (L., 1767) (Polychaeta, Phyllodocidae)
Ischnochiton hakodadensis Carpenter, 1893 (Mollusca: Polyplacophora)

Preparations

Preparation IBR-LPN-LALM-0-1

Larvae of annelid Phyllodoce maculata (Polychaeta, Phyllodocidae). Free swimming planktotrophic larvae, 10h after hatching.

Species

Phyllodoce maculata (Linnaeus, 1767)

Taxon

Annelida

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Description: Whole mount preparation of a phyllodoce larvae, view from the mouth opening (frontal view).

Staining: acetylated alpha-tubulin, serotonin

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Preparation IBR-LPN-LALM-0-2

Larvae of annelid Phyllodoce maculata (Polychaeta, Phyllodocidae) after hatching. Appearance of the pioneer neurons.

Species

Phyllodoce maculata (Linnaeus, 1767)

Taxon

Annelida

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Description: Whole mount preparation of phyllodoce larvae at the stage of pioneer neurons appearance.

Staining: acetylated alpha-tubulin, serotonin

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Preparation IBR-LPN-LALM-0-3

Larvae of chiton Ischnochiton hakodadensis (Mollusca: Polyplacophora), 20 h development.

Species

Ischnochiton hakodadensis Carpenter, 1893

Taxon

Polyplacophora

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Description: Whole mount preparation of a chiton larvae, ventral view.

Staining: acetylated alpha-tubulin, serotonin

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Preparation IBR-LPN-LALM-0-4

Lymnaea stagnalis (Basommatophora, Lymnaeidae) veliger

Species

Lymnaea stagnalis (Linnaeus, 1758)

Taxon

Gastropoda

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Description: Solitary larva, lateral view.

Staining: acetylated alpha-tubulin, FMRFamide, nuclear staining with DAPI

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Preparation IBR-LPN-LALM-0-5

Trochophore of an annelid Platynereis dumerilii (Polychaeta, Nereididae). View from the apical organ.

Species

Platynereis dumerilii Audouin & Milne Edwards, 1834

Taxon

Annelida

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Description: Solitary larva.

Staining: acetylated alpha-tubulin, FMRFamide, serotonin

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Preparation IBR-LPN-LALM-0-6

Helisoma trivolvis (Mollusca, Gastropoda) veliger. Frontal view.

Species

Helisoma trivolvis (Say, 1817)

Taxon

Gastropoda

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Description: Solitary larva, frontal view.

Staining: acetylated alpha-tubulin, serotonin

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Preparation IBR-LPN-LALM-0-7

Nervous system of an oyster veliger Crassostrea gigas (Mollusca, Bivalvia).

Species

Crassostrea gigas (Thunberg, 1793)

Taxon

Bivalvia

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Description: Whole mount of a Crassostrea gigas veliger, stained with antibodies against 5HT, FMRF-amide and α-tubulin, 36 hours after fertilisation.

Staining: acetylated alpha-tubulin, FMRFamide, serotonin

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