Investigation of the cardiac activity of three species of Bivalvia (Mytilus edulis L., Modiolus modiolus L. and Hiatella arctica L.) under salinity changes

I.N. Bakhmet, V.Ja. Berger, A.J. Komendantov, A.O. Smurov and V.V. Khalaman

Proceedings of the Zoological Institute RAS, 2013, 317(Supplement 3):

Abstract

In the manuscript the comparative analysis of three species of Bivalvia – Mytilus edulis, 1758, Modiolus modiolu, 1758 and Hiatella arctica, 1767 – under salinity changes was carried out. For the first time the registration of mussels cardiac activity to trace the reaction of organism was applied. Five types of salinity – 15, 20, 25, 30 and 35‰ – were used. The initial response to salinity change was a significant heart rate reduction with the exception of M. modiolus. In this species the increased cardiac activity was shown at high salinities. Change of salinity to the initial level to track the process of reacclimation led to sharp increase of cardiac activity. At the same time in M. modiolus the prominent bradycardia was marked. The results of investigations are discussed with respect to evolutionary history of the species.

Key words

acclimation, reacclimation, cardiac activity, salinity, Bivalvia

Published June 21, 2013

References

Бергер В.Я. 1986. Адаптации морских моллюсков к изменению солености salinity changes. Ленинград: Наука, 214 с.

Говберг Л.И. 1960. Распределение моллюсков в осадочных породах Онежского залива. Океанология, 8: 666–679.

Наумов А.Д. 2006. Двустворчатые моллюски Белого моря. Опыт эколого-фаунистического анализа. С.-Петербург: ЗИН РАН, 367 с.

Федяков В.В. 1988. Двустворчатые моллюски Соностровского района (Белое море, Кандалакшский залив). А.Н. Голиков (ред.). Экосистемы бентоса юго-восточной части Кандалакшского залива и прилегающих вод Белого моря. Исследование фауны морей, 38(46): 142–162.

Филиппов А.А., Комендантов А.Ю. и Халаман В.В. 2003. Соленостная толерантность беломорского моллюска Hiatella arctica (Bivalvia, Heterodonta). Зоологический журнал, 82: 913–918.

Хлебович В.В. 1974. Критическая соленость биологических процессов. Наука: Ленинград, 236 с.

Хлебович В.В. 1981. Акклимация животных организмов. Наука: Ленинград, 136 с.

Хлебович В.В., Яковишина Л.А. и Комендантов А.Ю. 1981. Изменение содержания электролитов в мантийной жидкости и гемолимфе беломорской мидии при длительном опреснении внешней среды. Биология моря, 2: 86–89.

Berger V.Ja. and Kharazova A.D. 1997. Mechanisms of salinity adaptations in marine molluscs. Hydrobiology, 355: 115–126. https://doi.org/10.1007/978-94-017-1907-0_12

Berger V.Ja. 2005. On the minimal terms of triggering the processes of phenotypic adaptation. Doklady Biological Sciences, 400: 57–60. https://doi.org/10.1007/s10630-005-0043-8

Braby C.E. and Somero G.N. 2006. Following the heart: temperature and salinity effects on heart rate in native and invasive species of blue mussels (genus Mytilus). Journal of Experimental Biology, 209: 2554–2566. https://doi.org/10.1242/jeb.02259

Camacho J., Qadri S.A., Wang H. and Worden M.K. 2006. Temperature acclimation alters cardiac performance in the lobster Homarus americanus. Journal of Comparative Physiology, A(192): 1327–1334. https://doi.org/10.1007/s00359-006-0162-1

Depledge M.H. and Andersen B.B. 1990. A computer-aided physiological monitoring system for continuous, long-term recording of cardiac activity in selected invertebrates. Comparative Biochemistry and Physiology, 96: 474–477. https://doi.org/10.1016/0300-9629(90)90664-E

Gardner J.P.A. and Thompson R.J. 2001. The effects of coastal and estuarine conditions on the physiology and survivorship of the mussels Mytilus edulis, M. trossulus and their hybrids. Journal of Experimental Marine Biology and Ecology, 265(2): 119–140. https://doi.org/10.1016/S0022-0981(01)00328-8

Graham J.H., Freeman D.C. and Emlen J.M. 1993. Developmental stability: a sensitive indicator of populations under stress. In: Landis W.G., Hughes J.S. and Lewis M.A. (eds) Environmental Toxicology Risk Assessment ASTM, Philadelphia: 137–168.

Pirro M.D., Cannicci S. and Santini G. 1999. A multi-factorial experiment on heart rate variations in the intertidal crab Pachygrapsus marmoratus. Marine Biology Research, 135(2): 341–345. https://doi.org/10.1007/s002270050632

Kinne O. 1967. Physiology of estuarine organisms with special reference to salinity and temperature: general aspects. In: Lauff G.H. (eds). Estuaries, American Association for the Advancement of Science, Washington: 525–540.

Leung B. and Forbes M. 1996. Fluctuating asymmetry in relation to stress and fitness: effect of trait type as revealed by meta-analysis. Ecoscience, 3: 400–413. https://doi.org/10.1080/11956860.1996.11682357

Neufeld D. and Wright S. 1998. Effect of cyclical salinity changes on cell volume and function in Geukensia demissa gills. Journal Experimental Biology, 201: 1421–1431. https://doi.org/10.1242/jeb.201.9.1421

Pierce S.K. 1970. Water balance in the genus Modiolus (Mollusca: Bivalvia: Mytilidae): osmotic concentrations in changing salinities. Comparative Biochemistry and Physiology, 36: 521–533. https://doi.org/10.1016/0010-406X(70)91028-5

Rovero F., Hughes R.N. and Chelazzi G. 1999. Cardiac and behavioural responses of mussels to risk of predation by dogwhelks. Animal Behaviour, 58(4): 707–714. https://doi.org/10.1006/anbe.1999.1176

Santini G., De Pirro M. and Chelazzi G. 1999. In situ and laboratory assessment of heart rate in a Mediterranean limpet using a noninvasive technique. Physiology and Biochemistry of Zoology, 72: 198–204. https://doi.org/10.1086/316656

Shumway S.E. 1977. Effect of salinity fluctuation on the osmotic pressure and Na+, Ca2+ and Mg2+ ion concentrations in the hemolymph of bivalve molluscs. Journal Marine Biology, 41: 153–177. https://doi.org/10.1007/BF00394023

Shumway S.E. and Youngson A. 1979. The effects of fluctuating salinity on the physiology of Modiolus demissus (Dillwyn). Journal of Experimental Marine Biology and Ecology, 40: 167–181. https://doi.org/10.1016/0022-0981(79)90043-1

Sukhotin A.A., Lajus D.L. and Lesin P.A. 2003. Influence of age and size on pumping activity and stress resistance in the marine bivalve Mytilus edulis L. Journal of Experimental Marine Biology and Ecology, 284: 129–144. https://doi.org/10.1016/S0022-0981(02)00497-5