Preparation

Nervous system of an oyster veliger Crassostrea gigas (Mollusca, Bivalvia).

In
Collection 24

Collection
Number

IBR-LPN-LALM-0-7

Species: Crassostrea gigas (Thunberg, 1793)    Bivalvia

Preparation Type: whole mount

Prepared: L.P. Nezlin, E.E. Voronezhskaya

Description: Whole mount of a Crassostrea gigas veliger, stained with antibodies against 5HT, FMRF-amide and α-tubulin, 36 hours after fertilisation.

Method: acetylated alpha-tubulin, FMRFamide, serotonin

Method Description: The staining method and protocol are described in detail in: Yurchenko O.V., Skiteva O., Voronezhskaya E. E., Dyachuk V.A. 2017. Nervous system development in the Pacific oyster Crassostrea gigas: comparative analysis of early neurogenesis in marine bivalves // Journal of Comparative Neurology. In press.

Institution: A.V. Zhirmunsky Institute of Marine Biology of the Far Eastern Branch RAS

 

 

 

Series
of images

Early larval neurons of an oyster veliger Crassostrea gigas (Mollusca, Bivalvia).

Preparation

IBR-LPN-LALM-0-7

Description:  5HT antibody marks neurons of the apical organ, FMRF-amid antibody - peripheral pioneer neurons, α-tubulin antibody - surface cilia.

Organs: larval nervous system

Methods: Immunohistochemistry - serotonin,
Immunohistochemistry - FMRFamide,
Immunohistochemistry - acetylated alpha-tubulin

Series Details...